{
    "accession": "MITE0000185",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-11-21",
            "contributors": [
                "0000-0001-6660-2715"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "Database ID crosslinks verified, added where possible; References properly formatted"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "FrsH",
        "description": "Non-heme diiron monooxygenase",
        "references": [
            "doi:10.1038/s41467-020-20418-3",
            "doi:10.1021/acschembio.3c00106"
        ],
        "databaseIds": {
            "uniprot": "A0A7S9SWM2",
            "genpept": "QPI18730.1",
            "mibig": "BGC0002125"
        },
        "auxiliaryEnzymes": [
            {
                "name": "FrsA",
                "description": "nonribosomal peptide synthetase",
                "databaseIds": {
                    "uniprot": "A0A7S9XGQ1",
                    "genpept": "QPI18723.1"
                }
            }
        ],
        "cofactors": {
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "beta-hydroxylation of PCP-bound leucine",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6@@:10](/[#8:22])-[#6:11](-[#7:13]-[#6:14]-[#6:15]-[#6:16](-[#7:18]-[#6:19]-[#6:20]-[#16:21]-[#6:29](=[#8:30])-[#6@@:27](\\[#7:28])-[#6:26]-[#6:24](-[#6:25])-[#6:23])=[#8:17])=[#8:12])(-[#6:4]-[#8:5]-[#15:6](-[#8:9])(-[#8:8])=[#8:7])-[#6:3]>>[#6:1]-[#6:2](-[#6@@:10](/[#8:22])-[#6:11](-[#7:13]-[#6:14]-[#6:15]-[#6:16](-[#7:18]-[#6:19]-[#6:20]-[#16:21]-[#6:29](=[#8:30])-[#6@@:27](\\[#7:28])-[#6:26](-[#8])-[#6:24](-[#6:25])-[#6:23])=[#8:17])=[#8:12])(-[#6:4]-[#8:5]-[#15:6](-[#8:8])(-[#8:9])=[#8:7])-[#6:3]",
            "reactions": [
                {
                    "substrate": "CC(C)C[C@H](N)C(=O)SCCNC(=O)CCNC(=O)[C@H](O)C(C)(C)COP(=O)(O)O",
                    "products": [
                        "CC(C)C(O)[C@H](N)C(=O)SCCNC(=O)CCNC(=O)[C@H](O)C(C)(C)COP(=O)(O)O"
                    ],
                    "isIntermediate": false,
                    "description": "beta-hydroxylation of PCP-bound leucine"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1038/s41467-020-20418-3",
                    "doi:10.1021/acschembio.3c00106"
                ]
            }
        }
    ]
}