{
    "accession": "MITE0000183",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-11-28",
            "contributors": [
                "0000-0002-4058-6718"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 added reaction databaseID crosslinks."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "EryK",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/bi00006a006",
            "doi:10.1111/j.1472-765X.2010.02973.x"
        ],
        "databaseIds": {
            "uniprot": "P48635",
            "genpept": "CAM00054.1",
            "mibig": "BGC0000055"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "C-12 hydroxylation of erythromycin B to form erythromycin A",
            "reactionSMARTS": "[#6:1]-[#6:2]\\[#6:3]1-[#8:18]-[#6:16](=[#8:17])-[#6:15](/[#6:19])-[#6:14](\\[#8:20]/[#6:21]2-[#8:26]-[#6:25](\\[#6:27])-[#6:24](/[#8:28])-[#6:23](-[#8:30]-[#6:31])(\\[#6:29])-[#6:22]-2)-[#6:13](/[#6:32])-[#6:12](\\[#8:33]/[#6:34]2-[#8:39]-[#6:38](/[#6:40])-[#6:37]-[#6:36](/[#7:41](-[#6:43])-[#6:42])-[#6:35]-2\\[#8:44])-[#6:11](-[#8:46])(\\[#6:45])-[#6:10]-[#6:9](\\[#6:47])-[#6:7](=[#8:8])-[#6:6](\\[#6:48])-[#6:5](\\[#8:49])-[#6:4]-1/[#6:50]>>[#6:1]-[#6:2]/[#6:3]1-[#6:4](-[#6:50])(\\[#8])-[#6:5](/[#8:49])-[#6:6](\\[#6:48])-[#6:7](=[#8:8])-[#6:9](/[#6:47])-[#6:10]-[#6:11](/[#6:45])(-[#8:46])-[#6:12](/[#8:33]\\[#6:34]2-[#6:35](/[#8:44])-[#6:36](\\[#7:41](-[#6:42])-[#6:43])-[#6:37]-[#6:38](\\[#6:40])-[#8:39]-2)-[#6:13](\\[#6:32])-[#6:14](\\[#8:20]\\[#6:21]2-[#6:22]-[#6:23](/[#6:29])(-[#8:30]-[#6:31])-[#6:24](\\[#8:28])-[#6:25](/[#6:27])-[#8:26]-2)-[#6:15](/[#6:19])-[#6:16](=[#8:17])-[#8:18]-1",
            "reactions": [
                {
                    "substrate": "CCC1OC(=O)[C@H](C)[C@@H](OC2C[C@](C)(OC)[C@H](O)[C@@H](C)O2)[C@@H](C)[C@H](OC2O[C@@H](C)C[C@@H](N(C)C)[C@@H]2O)[C@@](C)(O)C[C@H](C)C(=O)[C@H](C)[C@H](O)[C@@H]1C",
                    "products": [
                        "CCC1OC(=O)[C@H](C)[C@@H](OC2C[C@](C)(OC)[C@H](O)[C@@H](C)O2)[C@@H](C)[C@H](OC2O[C@@H](C)C[C@@H](N(C)C)[C@@H]2O)[C@@](C)(O)C[C@H](C)C(=O)[C@H](C)[C@H](O)[C@]1(C)O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1111/j.1472-765X.2010.02973.x"
                ]
            }
        },
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "C-12 hydroxylation of erythromycin D to form erythromycin C",
            "reactionSMARTS": "[#6:1]-[#6:2]\\[#6:3]1-[#8:18]-[#6:16](=[#8:17])-[#6:15](/[#6:19])-[#6:14](\\[#8:20]/[#6:21]2-[#8:26]-[#6:25](\\[#6:27])-[#6:24](/[#8:28])-[#6:23](-[#8:30])(\\[#6:29])-[#6:22]-2)-[#6:13](/[#6:31])-[#6:12](\\[#8:32]/[#6:33]2-[#8:38]-[#6:37](/[#6:39])-[#6:36]-[#6:35](/[#7:40](-[#6:42])-[#6:41])-[#6:34]-2\\[#8:43])-[#6:11](-[#8:45])(\\[#6:44])-[#6:10]-[#6:9](\\[#6:46])-[#6:7](=[#8:8])-[#6:6](\\[#6:47])-[#6:5](\\[#8:48])-[#6:4]-1/[#6:49]>>[#6:1]-[#6:2]/[#6:3]1-[#6:4](-[#6:49])(\\[#8])-[#6:5](/[#8:48])-[#6:6](\\[#6:47])-[#6:7](=[#8:8])-[#6:9](/[#6:46])-[#6:10]-[#6:11](/[#6:44])(-[#8:45])-[#6:12](/[#8:32]\\[#6:33]2-[#6:34](/[#8:43])-[#6:35](\\[#7:40](-[#6:42])-[#6:41])-[#6:36]-[#6:37](\\[#6:39])-[#8:38]-2)-[#6:13](\\[#6:31])-[#6:14](\\[#8:20]\\[#6:21]2-[#6:22]-[#6:23](/[#6:29])(-[#8:30])-[#6:24](\\[#8:28])-[#6:25](/[#6:27])-[#8:26]-2)-[#6:15](/[#6:19])-[#6:16](=[#8:17])-[#8:18]-1",
            "reactions": [
                {
                    "substrate": "CCC1OC(=O)[C@H](C)[C@@H](OC2C[C@](C)(O)[C@H](O)[C@@H](C)O2)[C@@H](C)[C@H](OC2O[C@@H](C)C[C@@H](N(C)C)[C@@H]2O)[C@@](C)(O)C[C@H](C)C(=O)[C@H](C)[C@H](O)[C@@H]1C",
                    "products": [
                        "CCC1OC(=O)[C@H](C)[C@@H](OC2C[C@](C)(O)[C@H](O)[C@@H](C)O2)[C@@H](C)[C@H](OC2O[C@@H](C)C[C@@H](N(C)C)[C@@H]2O)[C@@](C)(O)C[C@H](C)C(=O)[C@H](C)[C@H](O)[C@]1(C)O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1111/j.1472-765X.2010.02973.x"
                ]
            },
            "databaseIds": {
                "rhea": "32631",
                "ec": "1.14.13.154"
            }
        }
    ]
}