{
    "accession": "MITE0000182",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-11-21",
            "contributors": [
                "0000-0002-8323-5416"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer 1 changed tailoring description from 'sulfation' to 'sulfonation'."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "SxtN",
        "description": "N-sulfotransferase",
        "references": [
            "doi:10.1021/acschembio.8b00608"
        ],
        "databaseIds": {
            "uniprot": "A0A3G2KSQ1",
            "genpept": "AYN62308.1"
        },
        "cofactors": {
            "organic": [
                "PAPS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Sulfonation"
            ],
            "description": "N-sulfonation of saxitoxin",
            "reactionSMARTS": "[#8:1]=[#6:2](-[#8:4]-[#6:5]\\[#6@:6]1-[#6@:10]2-[#6@:17]3(\\[#6:16](-[#8:20])(-[#8:19])-[#6:15]-[#6:14]-[#7:21]-3-[#6:8](-[#7:9])=[#7:7]-1)/[#7:13]-[#6:12](-[#7:18])=[#7:11]/2)-[#7:3]>>[#8:1]=[#6:2](-[#8:4]-[#6:5]\\[#6@:6]1-[#6@:10]2-[#6@:17]3(\\[#6:16](-[#8:19])(-[#8:20])-[#6:15]-[#6:14]-[#7:21]-3-[#6:8](-[#7:9])=[#7:7]-1)/[#7:13]-[#6:12](-[#7:18])=[#7:11]/2)-[#7:3]-[#16](-[#8])(=[#8])=[#8]",
            "reactions": [
                {
                    "substrate": "NC(=O)OC[C@@H]1N=C(N)N2CCC(O)(O)[C@@]23NC(N)=N[C@@H]13",
                    "products": [
                        "NC1=N[C@H]2[C@H](COC(=O)NS(=O)(=O)O)N=C(N)N3CCC(O)(O)[C@]23N1"
                    ],
                    "isIntermediate": false,
                    "description": "N-21 sulfonation of saxitoxin to produce Gonyautoxin 5"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acschembio.8b00608"
                ]
            }
        }
    ]
}