{
    "accession": "MITE0000180",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-11-19",
            "contributors": [
                "0000-0001-7044-2419"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New Entry. Reviewer 1 changed databaseID references to fit strain described in publication, added 4-phosphopantetheine cofactor, added alternative reaction using leucine instead of phenylalanine."
        },
        {
            "version": "2",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        },
        {
            "version": "3",
            "date": "2026-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Replaced retired UniProtKB ID with UniParc ID."
        }
    ],
    "enzyme": {
        "name": "SLI_0884",
        "description": "Leucyl/Phenylalanine-tRNA-protein transferase",
        "references": [
            "doi:10.1093/jimb/kuad021",
            "doi:10.1093/gbe/evt082"
        ],
        "databaseIds": {
            "uniprot": "UPI0003430BF9",
            "genpept": "EOY45603.1",
            "mibig": "BGC0001168"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "amide bond formation between arginine, encoded by the A domain of the NRPS-like gene, and phenylalanine provided by their cognate aminoacyl-tRNAs as substrate.",
            "reactionSMARTS": "[#7:1]-[#6:2](=[#7:4]-[#6:5]-[#6:6]-[#6:7]-[#6@@:8](-[#6:10](-[#16:12]-[#6:32]-[#6:31]-[#7:30]-[#6:28](=[#8:29])-[#6:27]-[#6:26]-[#7:25]-[#6:23](=[#8:24])-[#6@:22](/[#8:33])-[#6:14](-[#6:16]-[#8:17]-[#15:18](-[#8:21])(-[#8:20])=[#8:19])(-[#6:15])-[#6:13])=[#8:11])/[#7:9])-[#7:3]>>[#7:1]-[#6:2](=[#7:4]-[#6:5]-[#6:6]-[#6:7]-[#6@:8](/[#7:9]-[#6](=[#8])-[#6@](-[#6]1:[#6]:[#6]:[#6]:[#6]:[#6]:1)\\[#7])-[#6:10](-[#16:12]-[#6:32]-[#6:31]-[#7:30]-[#6:28](=[#8:29])-[#6:27]-[#6:26]-[#7:25]-[#6:23](=[#8:24])-[#6@:22](/[#8:33])-[#6:14](-[#6:16]-[#8:17]-[#15:18](-[#8:21])(-[#8:20])=[#8:19])(-[#6:13])-[#6:15])=[#8:11])-[#7:3]",
            "reactions": [
                {
                    "substrate": "CC(C)(COP(=O)(O)O)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)[C@@H](N)CCCN=C(N)N",
                    "products": [
                        "CC(C)(COP(=O)(O)O)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)c1ccccc1"
                    ],
                    "isIntermediate": false,
                    "description": "dipeptide formation arg-phe"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1093/jimb/kuad021",
                    "doi:10.1093/gbe/evt082"
                ]
            }
        },
        {
            "tailoring": [
                "Other"
            ],
            "description": "amide bond formation between arginine, encoded by the A domain of the NRPS-like gene, and leucine provided by their cognate aminoacyl-tRNAs as substrate.",
            "reactionSMARTS": "[#7:1]-[#6:2](=[#7:4]-[#6:5]-[#6:6]-[#6:7]-[#6@@:8](-[#6:10](-[#16:12]-[#6:32]-[#6:31]-[#7:30]-[#6:28](=[#8:29])-[#6:27]-[#6:26]-[#7:25]-[#6:23](=[#8:24])-[#6@:22](/[#8:33])-[#6:14](-[#6:16]-[#8:17]-[#15:18](-[#8:21])(-[#8:20])=[#8:19])(-[#6:15])-[#6:13])=[#8:11])/[#7:9])-[#7:3]>>[#7:1]-[#6:2](=[#7:4]-[#6:5]-[#6:6]-[#6:7]-[#6@:8](/[#7:9]-[#6](=[#8])-[#6@@](\\[#7])-[#6]-[#6](-[#6])-[#6])-[#6:10](-[#16:12]-[#6:32]-[#6:31]-[#7:30]-[#6:28](=[#8:29])-[#6:27]-[#6:26]-[#7:25]-[#6:23](=[#8:24])-[#6@:22](/[#8:33])-[#6:14](-[#6:16]-[#8:17]-[#15:18](-[#8:21])(-[#8:20])=[#8:19])(-[#6:13])-[#6:15])=[#8:11])-[#7:3]",
            "reactions": [
                {
                    "substrate": "CC(C)(COP(=O)(O)O)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)[C@@H](N)CCCN=C(N)N",
                    "products": [
                        "CC(C)C[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)SCCNC(=O)CCNC(=O)[C@H](O)C(C)(C)COP(=O)(O)O"
                    ],
                    "isIntermediate": false,
                    "description": "dipeptide formation arg-leu"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1093/jimb/kuad021",
                    "doi:10.1093/gbe/evt082"
                ]
            }
        }
    ]
}