{
    "accession": "MITE0000176",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-11-14",
            "contributors": [
                "0000-0001-6585-0696"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer 1 checked for factual correctness of submission incl. references and database ID, added reaction information."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "Ple1",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1038/s41467-017-01659-1",
            "doi:10.1002/cbic.201700434",
            "doi:10.1038/srep25202"
        ],
        "databaseIds": {
            "uniprot": "A0A6S6QI82",
            "genpept": "BCI98769.1",
            "mibig": "BGC0002181"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylation, Ple1 catalyzed hydroxylation on the α position of the acetyl side chain",
            "reactionSMARTS": "[#6:1]/[#6@:2]1(-[#6:26]-[#6@@:21](/[#8:22]-[#6:23](=[#8:25])-[#6:24])-[#6@:19]2(\\[#6:20])-[#6@:12](/[#6:13])-[#6:11]-[#6:10]\\[#6@:9]3(-[#6:17]-[#6:16]-[#6:15](=[#8:18])\\[#6@:14]-3-2)-[#6@@:7](/[#6:8])-[#6@@:5]-1\\[#8:6])-[#6:3]=[#6:4]>>[#6:1]/[#6@:2]1(-[#6:26]-[#6@@:21](/[#8:22]-[#6:23](=[#8:25])-[#6:24]-[#8])-[#6@:19]2(\\[#6:20])-[#6@:12](/[#6:13])-[#6:11]-[#6:10]\\[#6@:9]3(-[#6:17]-[#6:16]-[#6:15](=[#8:18])\\[#6@:14]-3-2)-[#6@@:7](/[#6:8])-[#6@@:5]-1\\[#8:6])-[#6:3]=[#6:4]",
            "reactions": [
                {
                    "substrate": "C=C[C@]1(C)C[C@@H](OC(C)=O)[C@]2(C)[C@H](C)CC[C@]3(CCC(=O)[C@H]32)[C@@H](C)[C@@H]1O",
                    "products": [
                        "C=C[C@]1(C)C[C@@H](OC(=O)CO)[C@]2(C)[C@H](C)CC[C@]3(CCC(=O)[C@H]32)[C@@H](C)[C@@H]1O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1038/s41467-017-01659-1",
                    "doi:10.1002/cbic.201700434"
                ]
            }
        }
    ]
}