{
    "accession": "MITE0000175",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-11-12",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer 1 checked entries, crosslinks, databases and references"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "BytO",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1016/j.chembiol.2020.11.009"
        ],
        "databaseIds": {
            "uniprot": "A0A891LWT6",
            "genpept": "QRM13814.1",
            "mibig": "BGC0002084"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Biaryl bond formation"
            ],
            "description": "Biaryl formation between Y1 and H3 of the tripeptide, cytochrome P450, Planomonospora strain ID82291",
            "reactionSMARTS": "[#7:3]-[#6:4](-[#6:5](=[#8:6])-[#7:7]-[#6:8](-[#6:9]-[#6:10]1:[#6:11]:[#6:12]:[#6:13](-[#8:14]):[#6:15]:[#6:16]:1)-[#6:17](=[#8:18])-[#7:19]-[#6:20](-[#6:21](-[#8:22])=[#8:23])-[#6:24]-[#6:25]1:[n;H]:[#6:27]:[#7:28]:[#6:29]:1)-[#6:30]-[#6:31]1:[#6:32]:[#6:33]:[#6:34](-[#8:35]):[#6:36]:[#6:37]:1>>[#7:3]-[#6:4]1-[#6:30]-[#6:31]2:[#6:37]:[#6:36](:[#6:34](-[#8:35]):[#6:33]:[#6:32]:2)-[#6:27]2:[#7:28]:[#6:29]:[#6:25](:[n;H]:2)-[#6:24]-[#6:20](-[#6:21](=[#8:23])-[#8:22])-[#7:19]-[#6:17](=[#8:18])-[#6:8](-[#6:9]-[#6:10]2:[#6:16]:[#6:15]:[#6:13](-[#8:14]):[#6:12]:[#6:11]:2)-[#7:7]-[#6:5]-1=[#8:6]",
            "reactions": [
                {
                    "substrate": "CSCCC(N)C(=O)NC(CCCNC(=N)N)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(Cc1cnc[nH]1)C(=O)O",
                    "products": [
                        "CSCCC(N)C(=O)NC(CCCNC(=N)N)C(=O)NC1Cc2ccc(O)c(c2)-c2ncc([nH]2)CC(C(=O)O)NC(=O)C(Cc2ccc(O)cc2)NC1=O"
                    ],
                    "isIntermediate": false,
                    "description": "Modifies biarylitide YYH precursor MRYYH"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1016/j.chembiol.2020.11.009"
                ]
            }
        }
    ]
}