{
    "accession": "MITE0000169",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-2992-1607",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 added ProsA as auxiliary enzyme."
        }
    ],
    "enzyme": {
        "name": "ProsC",
        "description": "Short-chain dehydrogenase/reductase",
        "references": [
            "doi:10.1073/pnas.2310522120"
        ],
        "databaseIds": {
            "genpept": "WPM83322.1",
            "mibig": "BGC0003094"
        },
        "auxiliaryEnzymes": [
            {
                "name": "ProsA",
                "description": "Cytochrome P450",
                "databaseIds": {
                    "genpept": "WPM83320.1"
                }
            }
        ]
    },
    "reactions": [
        {
            "tailoring": [
                "Reduction"
            ],
            "description": "ProsC works in concert with P450 ProsA to yield the pathway intermediate. Order of reactions is not clear from experimental data. Overexpression of either ProsA or ProsC alone does not yield any product.",
            "reactionSMARTS": "[#6:5]-[c:9]1[c:8][c:7](-[#8:4])[c:11](-[#6:6])[c:12]2-[#8:13]-[#6:15](=[O:14])-[c:20]3[c:25](-[#6:17])[c:24][c:23](-[#8:19])[c:22](-[#6:16])[c:21]3-[#8:18]-[c:10]12>>[#6:6]-[#6:11]-1=[#6:12]-2-[#8:13]-[#6:15](=[O:14])-[c:20]3[c:25](-[#6:17])[c:24][c:23](-[#8:19])[c:22](-[#6:16])[c:21]3-[#8:18]-[#6@@H:10]-2[C@@:9]([#6:5])([#8])[#6@H:8](-[#8])-[#6:7]-1=[O:4] ",
            "reactions": [
                {
                    "substrate": "Cc1cc(O)c(C)c2c1Oc1c(C)c(O)cc(C)c1C(=O)O2",
                    "products": [
                        "CC1=C2OC(=O)c3c(C)cc(O)c(C)c3O[C@@H]2[C@@](C)(O)[C@H](O)C1=O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.2310522120"
                ]
            }
        }
    ]
}