{
    "accession": "MITE0000168",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-2992-1607",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 added ProsC as auxiliary enzyme."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "ProsA",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1073/pnas.2310522120"
        ],
        "databaseIds": {
            "genpept": "WPM83320.1",
            "mibig": "BGC0003094"
        },
        "auxiliaryEnzymes": [
            {
                "name": "ProsC",
                "description": "Short-chain dehydrogenase/reductase",
                "databaseIds": {
                    "genpept": "WPM83322.1"
                }
            }
        ],
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "ProsA works in concert with SDR ProsC to yield the pathway intermediate. Order of reactions is not clear from experimental data. Overexpression of either ProsA or ProsC alone does not yield any product.",
            "reactionSMARTS": "[#6:5]-[c:9]1[c:8][c:7](-[#8:4])[c:11](-[#6:6])[c:12]2-[#8:13]-[#6:15](=[O:14])-[c:20]3[c:25](-[#6:17])[c:24][c:23](-[#8:19])[c:22](-[#6:16])[c:21]3-[#8:18]-[c:10]12>>[#6:6]-[#6:11]-1=[#6:12]-2-[#8:13]-[#6:15](=[O:14])-[c:20]3[c:25](-[#6:17])[c:24][c:23](-[#8:19])[c:22](-[#6:16])[c:21]3-[#8:18]-[#6@@H:10]-2[C@@:9]([#6:5])([#8])[#6@H:8](-[#8])-[#6:7]-1=[O:4] ",
            "reactions": [
                {
                    "substrate": "Cc1cc(O)c(C)c2c1Oc1c(C)c(O)cc(C)c1C(=O)O2",
                    "products": [
                        "CC1=C2OC(=O)c3c(C)cc(O)c(C)c3O[C@@H]2[C@@](C)(O)[C@H](O)C1=O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.2310522120"
                ]
            }
        }
    ]
}