{
    "accession": "MITE0000166",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-2992-1607",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 added descriptions."
        },
        {
            "version": "2",
            "date": "2026-02-05",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Fixed typo in reaction description"
        }
    ],
    "enzyme": {
        "name": "ProsF",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1073/pnas.2310522120"
        ],
        "databaseIds": {
            "genpept": "WPM83325.1",
            "mibig": "BGC0003094"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "SAM (S-adenosyl-L-methionine)-dependent O-methylation of roseopurpurin intermediate.",
            "reactionSMARTS": "[#6:6]-[#6:11]-1=[#6:12]-2-[#8:13]-[#6:15](=[O:14])-[c:20]3[c:25](-[#6:17])[c:24][c:23](-[#8:19])[c:22](-[#6:16])[c:21]3-[#8:18]-[#6@@H:10]-2[C@@:9]([#6:5])([#8:27])[#6@H:8](-[#8:26])-[#6:7]-1=[O:4]>>[#6]-[#8:27][C@@:9]1([#6:5])[#6@H:8](-[#8:26])-[#6:7](=[O:4])-[#6:11](-[#6:6])=[#6:12]-2-[#8:13]-[#6:15](=[O:14])-[c:20]3[c:25](-[#6:17])[c:24][c:23](-[#8:19])[c:22](-[#6:16])[c:21]3-[#8:18]-[#6@H:10]1-2 ",
            "reactions": [
                {
                    "substrate": "CC1=C2OC(=O)c3c(C)cc(O)c(C)c3O[C@@H]2[C@@](C)(O)[C@H](O)C1=O",
                    "products": [
                        "CO[C@@]1(C)[C@H](O)C(=O)C(C)=C2OC(=O)c3c(C)cc(O)c(C)c3O[C@@H]21"
                    ],
                    "isIntermediate": false,
                    "description": "Yields roseopurpurin C and its C-4 epimer roesopurpurin D as the last step of the biosynthetic cascade."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.2310522120"
                ]
            }
        }
    ]
}