{
    "accession": "MITE0000155",
    "status": "active",
    "comment": "Acts on phenolic lipids to yield alkylresorcinol methyl ethers",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-4129-2890"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0003-0443-9902"
            ],
            "comment": "Initial entry. Reviewer partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer 2 replaced frequency variation with enumeration and fixed reaction numbering, reaction passes. "
        },
        {
            "version": "2",
            "date": "2024-12-09",
            "contributors": [
                "0000-0002-9747-3423"
            ],
            "reviewers": [
                "0000-0003-1763-2525"
            ],
            "comment": "included reaction description. Reviewer 1 corrected the SMARTS codes and SMILES. There were some carbons too much or too little in the acyl side chain."
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "SrsB",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1039/c9ob00807a"
        ],
        "databaseIds": {
            "genpept": "BAG12889.1",
            "mibig": "BGC0003089"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "O-methylation of alkylresorcinols to yield alkylresorcinol methyl ethers",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6:3](-[#6:4])-[#6:5]-[#6:6]-[#6:7]-[#6:8]-[#6:9]-[#6:10]-[#6:11]-[#6:12]-[#6:13]-[#6:14]-[#6:15]-[#6:16]-[c:19]1[c:20][c:21](-[#8:22])[c:23][c:24](-[#8:25])[c:26]1-[#6:27]>>[#6:1]-[#6:2]-[#6:3](-[#6:4])-[#6:5]-[#6:6]-[#6:7]-[#6:8]-[#6:9]-[#6:10]-[#6:11]-[#6:12]-[#6:13]-[#6:14]-[#6:15]-[#6:16]-[c:19]1[c:20][c:21](-[#8:22]-[#6])[c:23][c:24](-[#8:25])[c:26]1-[#6:27]",
            "reactions": [
                {
                    "substrate": "CCC(CCCCCCCCCCCCc1c(C)c(O)cc(O)c1)C",
                    "products": [
                        "CCC(CCCCCCCCCCCCc1c(C)c(O)cc(OC)c1)C"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1074/jbc.M710461200"
                ]
            }
        },
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "O-methylation of alkylresorcinols to yield alkylresorcinol methyl ethers",
            "reactionSMARTS": "[#6:2]-[#6:3](-[#6:4])-[#6:5]-[#6:6]-[#6:7]-[#6:8]-[#6:9]-[#6:10]-[#6:11]-[#6:12]-[#6:13]-[#6:14]-[#6:15]-[#6:16]-[#6:17]-[c:18]1[c:19][c:20](-[#8:21])[c:22][c:23](-[#8:24])[c:25]1-[#6:26]>>[#6]-[#8:21]-[c:20]1[c:22][c:23](-[#8:24])[c:25](-[#6:26])[c:18](-[#6:17]-[#6:16]-[#6:15]-[#6:14]-[#6:13]-[#6:12]-[#6:11]-[#6:10]-[#6:9]-[#6:8]-[#6:7]-[#6:6]-[#6:5]-[#6:3](-[#6:2])-[#6:4])[c:19]1",
            "reactions": [
                {
                    "substrate": "CC(CCCCCCCCCCCCCc1c(C)c(O)cc(O)c1)C",
                    "products": [
                        "CC(CCCCCCCCCCCCCc1c(C)c(O)cc(OC)c1)C"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1074/jbc.M710461200"
                ]
            }
        },
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "O-methylation of alkylresorcinols to yield alkylresorcinol methyl ethers",
            "reactionSMARTS": "[#6:24]-[#6:23](-[#6:25])-[#6:21]-[#6:20]-[#6:19]-[#6:18]-[#6:17]-[#6:16]-[#6:15]-[#6:14]-[#6:13]-[#6:12]-[#6:11]-[#6:10]-[c:9]1[c:8][c:6](-[#8:7])[c:5][c:3](-[#8:4])[c:2]1-[#6:1]>>[#6]-[#8:7]-[c:6]1[c:5][c:3](-[#8:4])[c:2](-[#6:1])[c:9](-[#6:10]-[#6:11]-[#6:12]-[#6:13]-[#6:14]-[#6:15]-[#6:16]-[#6:17]-[#6:18]-[#6:19]-[#6:20]-[#6:21]-[#6:23](-[#6:24])-[#6:25])[c:8]1",
            "reactions": [
                {
                    "substrate": "CC(CCCCCCCCCCCCc1c(C)c(O)cc(O)c1)C",
                    "products": [
                        "CC(CCCCCCCCCCCCc1c(C)c(O)cc(OC)c1)C"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1074/jbc.M710461200"
                ]
            }
        },
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "O-methylation of alkylresorcinols to yield alkylresorcinol methyl ethers",
            "reactionSMARTS": "[#6:24]-[#6:22](-[#6:23])-[#6:20]-[#6:19]-[#6:18]-[#6:17]-[#6:16]-[#6:15]-[#6:14]-[#6:13]-[#6:12]-[#6:11]-[#6:10]-[c:9]1[c:8][c:6](-[#8:7])[c:5][c:3](-[#8:4])[c:2]1-[#6:1]>>[#6]-[#8:7]-[c:6]1[c:5][c:3](-[#8:4])[c:2](-[#6:1])[c:9](-[#6:10]-[#6:11]-[#6:12]-[#6:13]-[#6:14]-[#6:15]-[#6:16]-[#6:17]-[#6:18]-[#6:19]-[#6:20]-[#6:22](-[#6:24])-[#6:23])[c:8]1",
            "reactions": [
                {
                    "substrate": "CC(CCCCCCCCCCCc1c(C)c(O)cc(O)c1)C",
                    "products": [
                        "CC(CCCCCCCCCCCc1c(C)c(O)cc(OC)c1)C"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1074/jbc.M710461200"
                ]
            }
        }
    ]
}