{
    "accession": "MITE0000126",
    "status": "active",
    "comment": "Capable of iodinating and brominating arenes and olefins",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0009-0006-9871-5187",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0003-0443-9902"
            ],
            "comment": "Initial entry. Reviewer 1 determined the entry as unfixable (multiple reactions). Reviewer 2 broke up multi-reaction into two distinct ones."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "AetF",
        "description": "Flavin-dependent halogenase",
        "references": [
            "doi:10.1021/jacs.1c12778"
        ],
        "databaseIds": {
            "uniprot": "A0A861B9Z9",
            "genpept": "QNL15176.1",
            "mibig": "BGC0002643"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Bromination of L-tryptophan, leading to 5-bromotryptophan",
            "reactionSMARTS": "[#7:1]-[#6:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:12]2:[#6:7](:[#6:8]:[#6:9]:[#6:10]:[#6:11]:2):[#7:6]:[#6:5]:1>>[#7:1]-[#6:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:12]2:[#6:7](:[#6:8]:[#6:9]:[#6:10](:[#6:11]:2)-[Br]):[#7:6]:[#6:5]:1",
            "reactions": [
                {
                    "substrate": "NC(Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "NC(Cc1c[nH]c2ccc(Br)cc12)C(=O)O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/jacs.1c12778"
                ]
            }
        },
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Bromination of 5-bromotryptophan, leading to 5,7-bromotryptophan",
            "reactionSMARTS": "[#7:1]-[#6:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:12]2:[#6:7](:[#6:8]:[#6:9]:[#6:10](:[#6:11]:2)-[Br:16]):[#7:6]:[#6:5]:1>>[#7:1]-[#6:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:12]2:[#6:7](:[#6:8](-[Br]):[#6:9]:[#6:10](:[#6:11]:2)-[Br:16]):[#7:6]:[#6:5]:1",
            "reactions": [
                {
                    "substrate": "NC(Cc1c[nH]c2ccc(Br)cc12)C(=O)O",
                    "products": [
                        "NC(Cc1c[nH]c2c(Br)cc(Br)cc12)C(=O)O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/jacs.1c12778"
                ]
            }
        }
    ]
}