{
    "accession": "MITE0000115",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0009-0001-3436-5302",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-0443-9902",
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer fixed reactionSMARTS:reactionSMARTS. Reviewer fixed reactions:products. Reviewer 2 changed enzyme and reaction description."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Replaced retired UniProtKB ID with UniParc ID. Removed spurious auxiliary Enzymes (referenced in MIBiG BGC)."
        }
    ],
    "enzyme": {
        "name": "NgnP2",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/acschembio.9b01034"
        ],
        "databaseIds": {
            "uniprot": "UPI00100569C8",
            "genpept": "AXG22415.1",
            "mibig": "BGC0002024"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Monooxygenation",
                "Oxidation"
            ],
            "description": "Hydroxylation at C-18",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6@H:3]1-[#8:4]-[#6:5](=[O:6])-[#6@@H:7](-[#8:8])-[#6:9]-[#6:10]-2-[#6:11]=[#6:12]-[#6:13]-3-[#6:14]-[#6@H:15](-[#8:16])-[#6@H:17](-[#6:18])-[#6@H:19](-[#8:20])-[#6:21]-3-[#6:22]-2\\[#6:23](-[#6:24])=[#6:25]\\[#6@H:26]-1-[#6:27]>>[#6:1]-[#6@H:2](-[#8:3])-[#6@H:4]1-[#8:5]-[#6:6](=[O:7])-[#6@@H:8](-[#8:9])-[#6:10]-[#6:11]-2-[#6:12]=[#6:13]-[#6:14]-3-[#6:15]-[#6@H:16](-[#8:17])-[#6@H:18](-[#6:19])-[#6@H:20](-[#8:21])-[#6:22]-3-[#6:23]-2\\[#6:24](-[#6:25])=[#6:26]\\[#6@H:27]-1-[#6]",
            "reactions": [
                {
                    "substrate": "CC[C@H]1OC(=O)[C@@H](O)CC2C=CC3C[C@H](O)[C@H](C)[C@H](O)C3C2/C(C)=C/[C@H]1C",
                    "products": [
                        "C/C1=C\\[C@@H](C)[C@@H]([C@H](C)O)OC(=O)[C@@H](O)CC2C=CC3C[C@H](O)[C@H](C)[C@H](O)C3C12"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acschembio.9b01034"
                ]
            }
        }
    ]
}