{
    "accession": "MITE0000114",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0009-0001-3436-5302",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0003-0443-9902",
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer partially fixed reactions:substrate, validation still not passing. Reviewer partially fixed reactions:products, validation still not passing. Reviewer 2 reworked reaction SMARTS, reaction passes. Reviewer 3 changed enzyme and reaction description."
        },
        {
            "version": "2",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        },
        {
            "version": "3",
            "date": "2025-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Replaced retired UniProtKB ID with UniParc ID. Removed spurious auxiliary Enzymes (referenced in MIBiG BGC)."
        }
    ],
    "enzyme": {
        "name": "NgnM",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1021/acschembio.9b01034"
        ],
        "databaseIds": {
            "uniprot": "UPI00100556BE",
            "genpept": "AXG22413.1",
            "mibig": "BGC0002024"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "NgnM methylates the hydroxyl group at the 2-position of the macrolactone intermediate",
            "reactionSMARTS": "[#6:1]/[#6@@:2](-[#6@@:4]1-[#6@:27](\\[#6:28])-[#6:26]=[#6:24](-[#6:25])/[#6@@:23]2-[#6@:11](-[#6:12]=[#6:13]/[#6@:14]3-[#6@:22]-2\\[#6@@:20](/[#8:21])-[#6@@:18](/[#6:19])-[#6@@:16](/[#8:17])-[#6:15]-3)\\[#6:10]-[#6@:8](\\[#8:9])-[#6:6](=[#8:7])-[#8:5]\\1)-[#8:3]>>[#6]-[#8:9]\\[#6@@:8]1-[#6:6](=[#8:7])-[#8:5]\\[#6@:4](-[#6@@:2](/[#8:3])-[#6:1])-[#6@:27](\\[#6:28])-[#6:26]=[#6:24](-[#6:25])/[#6@@:23]2-[#6@:11](-[#6:12]=[#6:13]/[#6@:14]3-[#6@:22]-2\\[#6@@:20](/[#8:21])-[#6@@:18](/[#6:19])-[#6@@:16](/[#8:17])-[#6:15]-3)\\[#6:10]-1",
            "reactions": [
                {
                    "substrate": "C/C1=C\\[C@@H](C)[C@@H]([C@H](C)O)OC(=O)[C@@H](O)C[C@H]2C=C[C@@H]3C[C@H](O)[C@H](C)[C@H](O)[C@H]3[C@H]12",
                    "products": [
                        "CO[C@H]1C[C@H]2C=C[C@@H]3C[C@H](O)[C@H](C)[C@H](O)[C@H]3[C@@H]2C(C)=C[C@@H](C)[C@@H]([C@H](C)O)OC1=O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acschembio.9b01034"
                ]
            }
        }
    ]
}