{
    "accession": "MITE0000110",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0009-0001-3436-5302",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0003-0443-9902"
            ],
            "comment": "Initial entry. Reviewer partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer partially fixed reactions:substrate, validation still not passing. Reviewer partially fixed reactions:products, validation still not passing. Reviewer 2 fixed pyrrole-nitrogen designation (explicit H), reaction passes."
        },
        {
            "version": "2",
            "date": "2025-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Replaced retired UniProtKB ID with UniParc ID. Removed spurious auxiliary Enzymes (referenced in MIBiG BGC)."
        }
    ],
    "enzyme": {
        "name": "NgnN4",
        "description": "Proline adenyltransferase",
        "references": [
            "doi:10.1021/acschembio.9b01034"
        ],
        "databaseIds": {
            "uniprot": "UPI00100571B0",
            "genpept": "AXG22420.1",
            "mibig": "BGC0002024"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "NgnN4 is responsible for attaching a pyrrole-moiety.",
            "reactionSMARTS": "[#6:1]-[#8:2]/[#6@@:3]1-[#6:26](=[#8:27])-[#8:25]/[#6@:24](-[#6@@:28](\\[#8:30])-[#6:29])-[#6@:22](/[#6:23])-[#6:21]=[#6:19](-[#6:20])-[#6@:11]23\\[#6@@:12]4-[#6@:13](-[#6@:15](-[#6@@:17](/[#8:18])-[#6@:9](\\[#8:10]/2)-[#6@@:8]-4/[#6:7]=[#6:6]-[#6@@:5]-3/[#6:4]-1)/[#6:16])/[#8:14]>>[#6:1]-[#8:2]/[#6@@:3]1-[#6:26](=[#8:27])-[#8:25]/[#6@:24](-[#6@@:28](\\[#8:30])-[#6:29])-[#6@:22](/[#6:23])-[#6:21]=[#6:19](-[#6:20])-[#6@:11]23\\[#6@@:12]4-[#6@:13](-[#6@:15](-[#6@@:17](/[#8:18]-[#6](-[#6]5:[nH]:[#6]:[#6]:[#6]:5)=[#8])-[#6@:9](\\[#8:10]/2)-[#6@@:8]-4/[#6:7]=[#6:6]-[#6@@:5]-3/[#6:4]-1)/[#6:16])/[#8:14]",
            "reactions": [
                {
                    "substrate": "CO[C@H]1C[C@H]2C=C[C@H]3[C@H]4O[C@]2(C(C)=C[C@@H](C)[C@@H]([C@H](C)O)OC1=O)[C@@H]3[C@@H](O)[C@@H](C)[C@H]4O",
                    "products": [
                        "CO[C@H]1C[C@H]2C=C[C@H]3[C@H]4O[C@]2(C(C)=C[C@@H](C)[C@@H]([C@H](C)O)OC1=O)[C@@H]3[C@@H](O)[C@@H](C)[C@H]4OC(=O)c1ccc[nH]1"
                    ],
                    "description": "Installation of pyrrole-moiety at the hydroxyl group at the 9-position of substrate",
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acschembio.9b01034"
                ]
            }
        }
    ]
}