{
    "accession": "MITE0000104",
    "status": "active",
    "comment": "Heterodimer TrlCD. Previously referenced retired MIBiG entry BGC0001487.",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0002-6077-7490",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-0443-9902"
            ],
            "comment": "Initial entry. Reviewer partially fixed general aromaticity."
        },
        {
            "version": "2",
            "date": "2025-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Removed retired MIBiG BGC."
        }
    ],
    "enzyme": {
        "name": "TrlC",
        "description": "Flavoprotein monooxygenase",
        "references": [
            "doi:10.1039/d4sc01715c"
        ],
        "databaseIds": {
            "uniprot": "A0A2S1JZG6",
            "genpept": "AWF83807.1"
        },
        "auxiliaryEnzymes": [
            {
                "name": "TrlD",
                "description": "Reductase component of flavoprotein monooxygenase TrlCD, reduces the flavin cofactor needed for enzyme catalysis",
                "databaseIds": {
                    "uniprot": "A0A2S1JZG9",
                    "genpept": "AWF83808.1"
                }
            }
        ]
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Catalyzes hydroxylation from 7-hydroxytropolone to 3,7-dihydroxytropolone",
            "reactionSMARTS": "[#8:1]-[c:2]1[c:3][c:4][c:5][c:6][c:7](-[#8:8])[c:9]1=[O:10]>>[#8:7]-[c:6]1[c:5][c:4][c:3][c:2](-[#8:1])[c:10](=[O])[c:8]1-[#8:9]",
            "reactions": [
                {
                    "substrate": "O=c1c(O)ccccc1O",
                    "products": [
                        "O=c1c(O)cccc(O)c1O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1039/d4sc01715c"
                ]
            }
        },
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Catalyzes hydroxylation from tropolone to 7-hydroxytropolone",
            "reactionSMARTS": "[#8:1]-[c:2]1[c:3][c:4][c:5][c:6][c:7][c:8]1=[O:9]>>[#8:1]-[c:2]1[c:3][c:4][c:5][c:6][c:7](-[#8:8])[c:9]1=[O]",
            "reactions": [
                {
                    "substrate": "O=c1cccccc1O",
                    "products": [
                        "O=c1c(O)ccccc1O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1039/d4sc01715c"
                ]
            }
        }
    ]
}