{
    "accession": "MITE0000095",
    "status": "active",
    "comment": "Previously referenced retired MIBiG entry BGC0001378.",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0003-0443-9902"
            ],
            "comment": "Initial entry. Reviewer partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer 2 reworked reaction SMARTS, reaction passing."
        },
        {
            "version": "2",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        },
        {
            "version": "3",
            "date": "2025-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Removed retired MIBiG BGC."
        }
    ],
    "enzyme": {
        "name": "TnmK1",
        "description": "Hydrolase",
        "references": [
            "doi:10.1021/jacs.2c08957"
        ],
        "databaseIds": {
            "uniprot": "A0A125SA14",
            "genpept": "AME18010.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization"
            ],
            "description": "Catalyzes D-ring-forming Michael addition",
            "reactionSMARTS": "[CH3:1][O:2][C:3]1[CH:33]2[CH:34]([CH:35]=[O:36])[C:7]([CH:8]([C:27]#[C:28][CH:29]=[CH:30][C:31]#[C:32]2)[NH:9][c:10]2[c:16]3[C:17]([c:19]4[c:24]([C:25](=[O:26])[c:15]3[c:13]([OH:14])[cH:12][cH:11]2)[cH:23][cH:22][cH:21][cH:20]4)=[O:18])=[C:5]([CH3:6])[CH:4]=1>>[CH3:1][O:2][C:3]1[CH:33]2[C:34]3([CH:11]4[CH2:12][C:13]([c:15]5[c:25]([OH:26])[c:24]6[c:19]([cH:20][cH:21][cH:22][cH:23]6)[c:17]([OH:18])[c:16]5[C:10]4=[N:9][CH:8]([C:27]#[C:28][CH:29]=[CH:30][C:31]#[C:32]2)[C:7]3=[C:5]([CH3:6])[CH:4]=1)=[O:14])[CH:35]=[O:36]",
            "reactions": [
                {
                    "substrate": "COC1=CC(C)=C2C(Nc3ccc(O)c4c3C(=O)c3ccccc3C4=O)C#CC=CC#CC1C2C=O",
                    "products": [
                        "COC1=CC(C)=C2C3C#CC=CC#CC1C2(C=O)C1CC(=O)c2c(c(O)c4ccccc4c2O)C1=N3"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Site-directed mutagenesis",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/jacs.2c08957"
                ]
            }
        }
    ]
}