{
    "accession": "MITE0000094",
    "status": "active",
    "comment": "Previously referenced retired MIBiG entry BGC0001378.",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 fixed reaction SMARTS."
        },
        {
            "version": "2",
            "date": "2025-07-03",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Removed retired MIBiG BGC."
        }
    ],
    "enzyme": {
        "name": "TnmK2",
        "description": "Iterative cofactorless dioxygenase",
        "references": [
            "doi:10.1038/s41589-023-01476-2"
        ],
        "databaseIds": {
            "uniprot": "A0A125SA15",
            "genpept": "AME18011.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dioxygenation"
            ],
            "reactionSMARTS": "[#6:1]-[#8:2]-[#6:3]1-[#6:15]2-[#6:16]3-[#6:17]4:[#6:23](-[#7:24]-[#6:8](-[#6:9]#[#6:10]-[#6:11]=[#6:12]-[#6:13]#[#6:14]-2)-[#6:7]-3=[#6:5](-[#6:6])-[#6:4]=1):[#6:22]1-[#6:25](-[#6:27]2:[#6:32](-[#6:33](=[#8:34])-[#6:21]:1:[#6:19](-[#8:20]):[#6:18]:4):[#6:31]:[#6:30]:[#6:29]:[#6:28]:2)=[#8:26]>>[#8:34]=[#6:33]1-[#6:32]2:[#6:31]:[#6:30]:[#6:29]:[#6:28]:[#6:27]:2-[#6:25](-[#6:22]2:[#6:21]-1:[#6:19](:[#6:18]:[#6:17]1-[#6:16]34-[#8]-[#6:7]-3(-[#6:5](=[#6:4]-[#6:3](=[#8])-[#8:2]-[#6:1])-[#6:6])-[#6:8](-[#6:9]#[#6:10]-[#6:11]=[#6:12]-[#6:13]#[#6:14]-[#6:15]-4-[#8])-[#7:24]-[#6:23]:1:2)-[#8:20])=[#8:26]",
            "reactions": [
                {
                    "substrate": "COC1=CC(C)=C2C3C#CC=CC#CC1C2c1cc(O)c2c(c1N3)C(=O)c1ccccc1C2=O",
                    "products": [
                        "COC(=O)C=C(C)C12OC13c1cc(O)c4c(c1NC2C#CC=CC#CC3O)C(=O)c1ccccc1C4=O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Site-directed mutagenesis",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41589-023-01476-2"
                ]
            }
        }
    ]
}