{
    "accession": "MITE0000091",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Initial entry. Reviewer 1 Changed evidence (the authors overexpressed only the terpene synthase gene, the rest is all inferred), filled enzyme type field, corrected substrate SMILES, adjusted descriptions and reaction SMARTS to represent the full reaction"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "CYP71BN1",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/ja508477e",
            "doi:10.1371/journal.pone.0119302"
        ],
        "databaseIds": {
            "uniprot": "K4CI56",
            "genpept": "AGK82807.1",
            "mibig": "BGC0001322"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "CYP71BN1 catalyzes the two-step oxidation of lycosantalene to lycosantalonol ",
            "reactionSMARTS": "[#6:1]-[#6:2](=[#6:4]-[#6:5]-[#6:6]-[#6:7](=[#6:9]-[#6:10]-[#6:11]-[#6:12]1(-[#6:19]2(-[#6:20])-[#6:16]3-[#6:17]-2-[#6:18]-[#6:14]-1-[#6:15]-3)-[#6:13])-[#6:8])-[#6:3]>>[#6:1]-[#6:2](=[#6:4]-[#6:5]-[#6:6]-[#6:7](-[#6:9](-[#6:10]-[#6:11]-[#6:12]1(-[#6:19]2(-[#6:20])-[#6:17]3-[#6:16]-2-[#6:15]-[#6:14]-1-[#6:18]-3)-[#6:13])=[#8])(-[#8])-[#6:8])-[#6:3]",
            "reactions": [
                {
                    "substrate": "CC(C)=CCC/C(C)=C\\CCC1(C)C2CC3C(C2)C31C",
                    "products": [
                        "CC(C)=CCCC(C)(O)C(=O)CCC1(C)C2CC3C(C2)C31C"
                    ],
                    "isIntermediate": true,
                    "description": "Two-step sequential oxidation of lycosantalene to lycosantalonol."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1021/ja508477e"
                ]
            }
        }
    ]
}