{
    "accession": "MITE0000089",
    "status": "retired",
    "retirementReasons": [
        "CPT2 is a gatekeeping enzyme producing the nerylneryl terpene scaffold and not a tailoring enzyme and therefore out of scope of MITE."
    ],
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 added correct number of products for a balanced reaction."
        },
        {
            "version": "2",
            "date": "2024-12-15",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Reviewer 1 retired the entry due to being out of scope of MITE."
        }
    ],
    "enzyme": {
        "name": "CPT2",
        "description": "Nerylneryl diphosphate synthase",
        "references": [
            "doi:10.1073/pnas.0904113106",
            "doi:10.1105/tpc.113.111013",
            "doi:10.1371/journal.pone.0119302"
        ],
        "databaseIds": {
            "uniprot": "K7WQ45",
            "genpept": "AGK82801.1",
            "mibig": "BGC0001322"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Prenylation"
            ],
            "reactionSMARTS": "[#6]\\[#6](-[#6])=[#6]\\[#6]-[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O.[#6]-[#6](=[#6])-[#6]-[#6]-[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O.[#6]-[#6](=[#6])-[#6]-[#6]-[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O.[#6]-[#6](=[#6])-[#6]-[#6]-[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O>>[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O.[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O.[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O.[#6]\\[#6](-[#6])=[#6]\\[#6]-[#6]\\[#6](-[#6])=[#6]/[#6]-[#6]\\[#6](-[#6])=[#6]/[#6]-[#6]\\[#6](-[#6])=[#6]/[#6]-[#8]P([#8-])(=O)[#8]P([#8-])([#8-])=O",
            "reactions": [
                {
                    "substrate": "C=C(C)CCOP(=O)([O-])OP(=O)([O-])[O-].C=C(C)CCOP(=O)([O-])OP(=O)([O-])[O-].C=C(C)CCOP(=O)([O-])OP(=O)([O-])[O-].CC(C)=CCOP(=O)([O-])OP(=O)([O-])[O-]",
                    "products": [
                        "O=P([O-])([O-])OP(=O)([O-])O",
                        "O=P([O-])([O-])OP(=O)([O-])O",
                        "O=P([O-])([O-])OP(=O)([O-])O",
                        "CC(C)=CCC/C(C)=C\\CC/C(C)=C\\CC/C(C)=C\\COP(=O)([O-])OP(=O)([O-])[O-]"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.0904113106"
                ]
            },
            "databaseIds": {
                "rhea": "11328",
                "ec": "2.5.1.28"
            }
        }
    ]
}