{
    "accession": "MITE0000064",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-3002-6458",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 reworked reaction SMARTS numbering and added descriptions."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "PtlI",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/ja0639214"
        ],
        "databaseIds": {
            "uniprot": "Q82IY3",
            "genpept": "BAC70710.1",
            "mibig": "BGC0000678"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "Catalyzes oxidation of pentalen-13-al in the neopentalenolactone pathway",
            "reactionSMARTS": "[#6:1]-[#6:2]1-[#6@:15]2-[#6@:10]3(-[#6@@:11](-[#6:13]-[#6:14]\\2)/[#6:12])-[#6:4](-[#6:5]-[#6:6](-[#6:9]/3)(-[#6:8])-[#6:7])-[#6:3]=1>>[#6:12]/[#6@:11]1-[#6@@:10]23-[#6@:15](-[#6:2](=[#6:3]-[#6:4]-2-[#6:5]-[#6:6](-[#6:9]/3)(-[#6:7])-[#6:8])-[#6:1]=[#8])\\[#6:14]-[#6:13]-1",
            "reactions": [
                {
                    "substrate": "CC1=CC2CC(C)(C)C[C@@]23[C@H](C)CC[C@@H]13",
                    "products": [
                        "C[C@@H]1CC[C@H]2C(C=O)=CC3CC(C)(C)C[C@]312"
                    ],
                    "isIntermediate": true,
                    "description": "Conversion of pentalenene to pentalen-13-al"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/ja0639214"
                ]
            },
            "databaseIds": {
                "rhea": "31699",
                "ec": "1.14.15.32"
            }
        }
    ]
}