{
    "accession": "MITE0000056",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0001-6534-6609",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer 1 changed text of enzyme description and enzyme reaction description, checked factual correctness and links"
        },
        {
            "version": "2",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "McjB",
        "description": "Peptidase",
        "references": [
            "doi:10.1002/cbic.201200016",
            "doi:10.1128/JB.181.8.2659-2662.1999",
            "doi:10.1002/cbic.201100596"
        ],
        "databaseIds": {
            "uniprot": "Q9X2V8",
            "genpept": "AAD28495.1",
            "mibig": "BGC0000581"
        },
        "auxiliaryEnzymes": [
            {
                "name": "McjC",
                "description": "ATP-dependent lactam synthetase",
                "databaseIds": {
                    "uniprot": "Q9X2V9",
                    "genpept": "AAD28496.1"
                }
            }
        ]
    },
    "reactions": [
        {
            "tailoring": [
                "Hydrolysis"
            ],
            "description": "ATP-dependent N-terminal cleavage of leader peptide, resulting in immature intermediate core peptide.",
            "reactionSMARTS": "[#6:1]-[#6@:32](\\[#7:33]-[#6:35](/[#6@:36](-[#6:37])-[#7:38]-[#6:40](-[#6@@:41]/[#7:42]-[#6:44](-[#6:45]-[#7:46]-[#6:48](/[#6@:167]-[#7:166]-[#6:165](=[#8:164])-[#6:163]-[#7:162]-[#6:161](=[#8:160])/[#6@@:159]-[#7:158]-[#6:157](=[#8:156])\\[#6@:155](-[#6:154])-[#7:153]-[#6:152](=[#8:151])\\[#6@:150](-[#6:149])-[#7:148]-[#6:147](=[#8:146])/[#6@:145](-[#6:144]-[#6:143]-[#6:142](=[#8:141])-[#8:140])-[#7:139]-[#6:138](=[#8:137])\\[#6@@:136]-[#7:135]-[#6:134](=[#8:133])\\[#6@@:132](-[#7:131]-[#6:130](=[#8:129])\\[#6@:128](-[#6:127])-[#7:126]-[#6:125](=[#8:124])-[#6:123]-[#7:122]-[#6:121](=[#8:120])-[#6@:119](\\[#6:118])-[#7:117]-[#6:116](=[#8:115])-[#6:114]-[#7:113]-[#6:112](=[#8:111])-[#6:110]-[#7:109]-[#6](=[#8])/[#6@](-[#6]-[#6]-[#6]-[#6]-[#7])-[#7]-[#6](=[#8])\\[#6@@](-[#7]-[#6](=[#8])/[#6@](-[#6]-[#6](-[#6])-[#6])-[#7]-[#6](=[#8])\\[#6@](-[#6]-[#6]-[#6](-[#7])=[#8])-[#7]-[#6](=[#8])/[#6@](-[#6]-[#8])-[#7]-[#6](=[#8])-[#6@](\\[#6])-[#7]-[#6](=[#8])/[#6@](-[#6]-[#8])-[#7]-[#6](=[#8])-[#6@@](/[#7])-[#6]-[#6]-[#6]-[#6]-[#7])-[#6@@](/[#6])-[#8])-[#6:49])=[#8:47])=[#8:43])=[#8:39])=[#8:34])-[#6:31](=[#8:30])-[#7:29]/[#6@@:28](-[#6:27])-[#6:26](=[#8:25])-[#7:24]\\[#6@@:23](-[#6:22]-[#6:21])-[#6:20](=[#8:19])-[#7:18]\\[#6@@:17](-[#6:16]-[#6:15]1:[#6:14]:[#6:13]:[#6:12](-[#8:11]):[#6:10]:[#6:9]:1)-[#6:8](=[#8:7])-[#7:6]-[#6:5]-[#6:4](=[#8:3])-[#8:2]>>[#6:1]-[#6@:32](\\[#7:33]-[#6:35](/[#6@:36](-[#6:37])-[#7:38]-[#6:40](-[#6@@:41]/[#7:42]-[#6:44](-[#6:45]-[#7:46]-[#6:48](/[#6@:167]-[#7:166]-[#6:165](=[#8:164])-[#6:163]-[#7:162]-[#6:161](=[#8:160])/[#6@@:159]-[#7:158]-[#6:157](=[#8:156])\\[#6@:155](-[#6:154])-[#7:153]-[#6:152](=[#8:151])\\[#6@:150](-[#6:149])-[#7:148]-[#6:147](=[#8:146])/[#6@:145](-[#6:144]-[#6:143]-[#6:142](=[#8:141])-[#8:140])-[#7:139]-[#6:138](=[#8:137])\\[#6@@:136]-[#7:135]-[#6:134](=[#8:133])\\[#6@@:132](-[#7:131]-[#6:130](=[#8:129])\\[#6@:128](-[#6:127])-[#7:126]-[#6:125](=[#8:124])-[#6:123]-[#7:122]-[#6:121](=[#8:120])-[#6@:119](\\[#6:118])-[#7:117]-[#6:116](=[#8:115])-[#6:114]-[#7:113]-[#6:112](=[#8:111])-[#6:110]-[#7:109])-[#6:49])=[#8:47])=[#8:43])=[#8:39])=[#8:34])-[#6:31](=[#8:30])-[#7:29]/[#6@@:28](-[#6:27])-[#6:26](=[#8:25])-[#7:24]\\[#6@@:23](-[#6:22]-[#6:21])-[#6:20](=[#8:19])-[#7:18]\\[#6@@:17](-[#6:16]-[#6:15]1:[#6:9]:[#6:10]:[#6:12](-[#8:11]):[#6:13]:[#6:14]:1)-[#6:8](=[#8:7])-[#7:6]-[#6:5]-[#6:4](=[#8:3])-[#8:2]",
            "reactions": [
                {
                    "substrate": "CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)CNC(=O)[C@H](C)NC(=O)CNC(=O)CNC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](N)CCSC)[C@@H](C)CC)C(C)C)C(C)C)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)C(C)C)C(C)C)C(=O)NCC(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)NCC(=O)O)[C@@H](C)CC)[C@@H](C)O",
                    "products": [
                        "CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)CNC(=O)[C@H](C)NC(=O)CNC(=O)CN)C(C)C)C(C)C)C(=O)NCC(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)NCC(=O)O)[C@@H](C)CC)[C@@H](C)O"
                    ],
                    "isIntermediate": true,
                    "description": "Precursor cleavage of the McjA precursor peptide, resulting in the intermediate core peptide before macrolactam formation."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1021/ja910191u",
                    "doi:10.1002/cbic.201200016"
                ]
            }
        }
    ]
}