{
    "accession": "MITE0000055",
    "status": "active",
    "comment": "Mismatch between protein sequences of UniProt Q9X2V9 and GenPept AAD28496.1 - UniProt sequence correct.",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0001-6534-6609",
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 adjusted core peptide recognition pattern to be more explicit."
        },
        {
            "version": "2",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "McjC",
        "description": "Macrolactam synthetase",
        "references": [
            "doi:10.1128/jb.181.8.2659-2662.1999",
            "doi:10.1002/cbic.201200016"
        ],
        "databaseIds": {
            "uniprot": "Q9X2V9",
            "genpept": "AAD28496.1",
            "mibig": "BGC0000581"
        },
        "auxiliaryEnzymes": [
            {
                "name": "McjB",
                "description": "Lassopeptide cysteine protease. Required for macrolactam-synthethase function (folds and cleaves precursor peptide)",
                "databaseIds": {
                    "uniprot": "Q9X2V8",
                    "genpept": "AAD28495.1"
                }
            }
        ]
    },
    "reactions": [
        {
            "tailoring": [
                "Macrolactam formation"
            ],
            "description": "Lassopeptide macrolactam formation after N-terminal cleavage: NH2-G-G-X-X-X-X-X-E-X-X-X-X-X-X-X-X-X-X-X-F-X-COOH.",
            "reactionSMARTS": "[#6:55]-[#6:54](-[#7:56]-[#6:57](=[O:58])-[#6&H2:59]-[#7:60]-[#6:61](=[O:62])-[#6&H2:63]-[#7&H2:64])-[#6:52](=[O:53])-[#7:51]-[#6:50]-[#6:48](=[O:49])-[#7:47]-[#6@@H:40](-[#6:41])-[#6:38](=[O:39])-[#7:37]-[#6@@H:36](-[#6:65])-[#6:34](=[O:35])-[#7:33]-[#6:32]-[#6:30](=[O:31])-[#7:29]-[#6@@H:23](-[#6:24]-[#6:25]-[#6:26](-[#8:28])=[O:27])-[#6:21](=[O:22])-[#7:20]-[#6@@H:18](-[#6:19])-[#6:16](=[O:17])-[#7:15]-[#6@@H:13](-[#6:14])-[#6:11](=[O:12])-[#7:10]-[#6:9]-[#6:7](=[O:8])-[#7:6]-[#6:5]-[#6:3](=[O:4])-[#7:2]-[#6:1]-[#6:66](=[O:67])-[#7:68]-[#6:69]-[#6:70](=[O:71])-[#7:72]-[#6:73]-[#6:74](=[O:75])-[#7:76]-[#6@@H:77](-[#6:111])-[#6:78](=[O:79])-[#7:80]-[#6@@H:81](-[#6:110])-[#6:82](=[O:83])-[#7:84]-[#6@@H:85](-[#6:86])-[#6:87](=[O:88])-[#7:89]-[#6@@H:90](-[#6:91])-[#6:92](=[O:93])-[#7:94]-[#6@@H:95](-[#6:96]-[c:97]1[c:98][c:99][c:100][c:101][c:102]1)-[#6:103](=[O:104])-[#7:105]-[#6:106]-[#6:107](-[#8:109])=[O:108]>>[#6:111]-[#6@H:77](-[#7:76]-[#6:74](=[O:75])-[#6:73]-[#7:72]-[#6:70](=[O:71])-[#6:69]-[#7:68]-[#6:66](=[O:67])-[#6:1]-[#7:2]-[#6:3](=[O:4])-[#6:5]-[#7:6]-[#6:7](=[O:8])-[#6:9]-[#7:10]-[#6:11](=[O:12])-[#6@H:13](-[#6:14])-[#7:15]-[#6:16](=[O:17])-[#6@H:18](-[#6:19])-[#7:20]-[#6:21](=[O:22])-[#6@@H:23]-1-[#6:24]-[#6:25]-[#6:26](=[O:27])-[#7&H1:64]-[#6&H2:63]-[#6:61](=[O:62])-[#7:60]-[#6&H2:59]-[#6:57](=[O:58])-[#7:56]-[#6:54](-[#6:55])-[#6:52](=[O:53])-[#7:51]-[#6:50]-[#6:48](=[O:49])-[#7:47]-[#6@@H:40](-[#6:41])-[#6:38](=[O:39])-[#7:37]-[#6@@H:36](-[#6:65])-[#6:34](=[O:35])-[#7:33]-[#6:32]-[#6:30](=[O:31])-[#7:29]-1)-[#6:78](=[O:79])-[#7:80]-[#6@@H:81](-[#6:110])-[#6:82](=[O:83])-[#7:84]-[#6@@H:85](-[#6:86])-[#6:87](=[O:88])-[#7:89]-[#6@@H:90](-[#6:91])-[#6:92](=[O:93])-[#7:94]-[#6@@H:95](-[#6:96]-[c:97]1[c:98][c:99][c:100][c:101][c:102]1)-[#6:103](=[O:104])-[#7:105]-[#6:106]-[#6:107](-[#8:109])=[O:108]",
            "reactions": [
                {
                    "substrate": "CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)CNC(=O)[C@H](C)NC(=O)CNC(=O)CN)C(C)C)C(C)C)C(=O)NCC(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)NCC(=O)O)[C@@H](C)CC)[C@@H](C)O",
                    "products": [
                        "CC[C@H](C)[C@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H]1CCC(=O)NCC(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](Cc2c[nH]cn2)C(=O)N[C@@H](C(C)C)C(=O)N2CCC[C@H]2C(=O)N1)C(C)C)C(=O)NCC(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)NCC(=O)O)[C@@H](C)CC)[C@@H](C)O"
                    ],
                    "isIntermediate": false,
                    "description": "Lasso macrocyclisation of microcin J25 after precursor cleavage, leading to mature product."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Site-directed mutagenesis",
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1074/jbc.M803995200"
                ]
            }
        }
    ]
}