{
    "accession": "MITE0000048",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer 1 removed parentheses around reaction SMARTS, reaction passes. Reviewer 2 switched text of enzyme description and enzyme reaction description."
        }
    ],
    "enzyme": {
        "name": "BotP",
        "description": "Peptidase",
        "references": [
            "doi:10.1039/d0np00097c",
            "doi:10.1016/j.chembiol.2012.08.013",
            "doi:10.1002/cbic.201600406"
        ],
        "databaseIds": {
            "uniprot": "K4MHW2",
            "genpept": "AFV25490.1",
            "mibig": "BGC0000469"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydrolysis"
            ],
            "description": "Cleavage of N-terminal Met from a NH2-Met-Gly-Pro-Xn chain, exposing a N-terminal Gly; bottromycin biosynthesis in Streptomyces sp. BC16019.",
            "reactionSMARTS": "[#6:1]-[#16:2]-[#6:3]-[#6:4]-[#6@H:5](-[#7H2:6])-[#6:7](=[O:8])-[#7:9]-[#6H2:10]-[#6:11](=[O:12])-[#7:13]-1-[#6:14]-[#6:15]-[#6:16]-[#6@H:17]-1-[#6:18]=[O:19]>>[#7H2:9]-[#6:10]-[#6:11](=[O:12])-[#7:13]-1-[#6:14]-[#6:15]-[#6:16]-[#6@H:17]-1-[#6:18]=[O:19]",
            "reactions": [
                {
                    "substrate": "CSCC[C@H](NC(=O)[C@H](CS)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@@H](N)CCSC)C(C)C)C(C)C)C(C)C)C(=O)O",
                    "products": [
                        "CSCC[C@H](NC(=O)[C@H](CS)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)CN)C(C)C)C(C)C)C(C)C)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Bottromycin A2-precursor peptide, first 10 AAs; cleaveage of first AA."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1002/cbic.201600406"
                ]
            }
        }
    ]
}