{
    "accession": "MITE0000047",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0003-0443-9902",
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer partially fixed reactionSMARTS:reactionSMARTS, validation still not passing. Reviewer partially fixed reactions:products, validation still not passing. Reviewer 2 fixed numbering issue in reaction SMARTS, reaction passes. Reviewer 3 switched text of enzyme description and enzyme reaction description."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        }
    ],
    "enzyme": {
        "name": "BotRMT3",
        "description": "C-methyltransferase",
        "references": [
            "doi:10.1039/C2SC21190D",
            "doi:10.1039/d0np00097c",
            "doi:10.1016/j.chembiol.2012.08.013"
        ],
        "databaseIds": {
            "uniprot": "K4MHG1",
            "genpept": "AFV25488.1",
            "mibig": "BGC0000469"
        },
        "cofactors": {
            "inorganic": [
                "FeS"
            ],
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Bottromycin-precursor acting rSAM methyltransferase from Streptomyces sp. BC16019;C-methylation of Pro2 of bottromycin A2.",
            "reactionSMARTS": "[#6:1]-[#16:2]-[#6:3]-[#6:4]/[#6@@:5](-[#6:64]=[#8:65])-[#7:6]-[#6:7](\\[#6@@:9](-[#7:12]-[#6:13](/[#6@@:15](-[#7:20]-[#6:21](/[#6@@:23](-[#7:31]-[#6:32](/[#6@:34](-[#6:61](-[#6:63])-[#6:62])-[#7:35]-[#6:36](\\[#6@:38](-[#6:58](-[#6:60])-[#6:59])-[#7:39]-[#6:40](/[#6@:42](-[#6:55](-[#6:57])-[#6:56])-[#7:43]-[#6:44](/[#6@:46]1-[#7:50](-[#6:51](-[#6:53]-[#7:54])=[#8:52])-[#6:49]-[#6:48]-[#6:47]-1)=[#8:45])=[#8:41])=[#8:37])=[#8:33])-[#6:24]-[#6:25]1:[#6:30]:[#6:29]:[#6:28]:[#6:27]:[#6:26]:1)=[#8:22])-[#6:16]-[#6:17](-[#8:19])=[#8:18])=[#8:14])-[#6:10]-[#16:11])=[#8:8]>>[#6:1]-[#16:2]-[#6:3]-[#6:4]/[#6@@:5](-[#6:64]=[#8:65])-[#7:6]-[#6:7](\\[#6@@:9](-[#7:12]-[#6:13](/[#6@@:15](-[#7:20]-[#6:21](/[#6@@:23](-[#7:31]-[#6:32](/[#6@:34](-[#6:61](-[#6:63])-[#6:62])-[#7:35]-[#6:36](\\[#6@:38](-[#6:58](-[#6:59])-[#6:60])-[#7:39]-[#6:40](/[#6@:42](-[#6:55](-[#6:56])-[#6:57])-[#7:43]-[#6:44](/[#6@:46]1-[#7:50](-[#6:51](-[#6:53]-[#7:54])=[#8:52])-[#6:49]-[#6:48]-[#6@@:47]-1/[#6])=[#8:45])=[#8:41])=[#8:37])=[#8:33])-[#6:24]-[#6:25]1:[#6:30]:[#6:29]:[#6:28]:[#6:27]:[#6:26]:1)=[#8:22])-[#6:16]-[#6:17](=[#8:18])-[#8:19])=[#8:14])-[#6:10]-[#16:11])=[#8:8]",
            "reactions": [
                {
                    "substrate": "CSCC[C@H](NC(=O)[C@H](CS)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)CN)C(C)C)C(C)C)C(C)C)C(=O)O",
                    "products": [
                        "CSCC[C@H](NC(=O)[C@H](CS)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H]1[C@@H](C)CCN1C(=O)CN)C(C)C)C(C)C)C(C)C)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Bottromycin A2-precursor peptide with cleaved N-terminal Met, first 10 AAs."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/d0np00097c"
                ]
            }
        }
    ]
}