{
    "accession": "MITE0000044",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0001-6534-6609",
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer fixed reaction SMARTS, reaction passes. Reviewer 2 switched text of enzyme description and enzyme reaction description."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "BotCYP",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/jacs.0c10361",
            "doi:10.1039/d0np00097c"
        ],
        "databaseIds": {
            "uniprot": "K4MJU2",
            "genpept": "AFV25487.2",
            "mibig": "BGC0000469"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Decarboxylation",
                "Oxidation"
            ],
            "description": "P450-catalyzed oxidative decarboxylation of terminal thiazoline of bottromycin precursor in Streptomyces sp. BC16019",
            "reactionSMARTS": "[#6:47]-[#6@H:18](\\[#7:17]=[#6:16]-1/[#7:15]-[#6:14]-[#6:12](=[O:13])-[#7:11]-2-[#6:10]-[#6:9]-[#6@@H:7](-[#6:8])-[#6@H:6]-2-[#6:4](=[O:5])-[#7:3]-[#6@@H:2](-[#6:1])-[#6:50](=[O:51])-[#7:49]-[#6@H:48]-1-[#6:52])-[#6:19](=[O:20])-[#7:21]-[#6@@H:22](-[#6@@H:23](-[#6:24])-[c:25]1[c:26][c:27][c:28][c:29][c:30]1)-[#6:31](=[O:32])-[#7:33]-[#6@H:34](-[#6:35]-[#6:36](-[#8:37])=[O:38])-[#6:39]-1=[#7:40]-[#6@@H:41](-[#6:42]-[#16:43]-1)-[#6:44](-[#8H:45])=[O:46]>>[#6:47]-[#6@H:18](\\[#7:17]=[#6:16]-1/[#7:15]-[#6:14]-[#6:12](=[O:13])-[#7:11]-2-[#6:10]-[#6:9]-[#6@@H:7](-[#6:8])-[#6@H:6]-2-[#6:4](=[O:5])-[#7:3]-[#6@@H:2](-[#6:1])-[#6:50](=[O:51])-[#7:49]-[#6@H:48]-1-[#6:52])-[#6:19](=[O:20])-[#7:21]-[#6@@H:22](-[#6@@H:23](-[#6:24])-[c:25]1[c:26][c:27][c:28][c:29][c:30]1)-[#6:31](=[O:32])-[#7:33]-[#6@H:34](-[#6:35]-[#6:36](-[#8:37])=[O:38])-[c:39]1[n:40][c:41][c:42][s:43]1",
            "reactions": [
                {
                    "substrate": "CC(C)[C@@H]1NC(=O)[C@@H]2[C@H](C)CCN2C(=O)CN/C(=N\\[C@H](C(=O)N[C@H](C(=O)N[C@H](CC(=O)O)C2=N[C@H](C(=O)O)CS2)[C@@H](C)c2ccccc2)C(C)(C)C)[C@H](C(C)(C)C)NC1=O",
                    "products": [
                        "CC(C)[C@@H]1NC(=O)[C@@H]2[C@H](C)CCN2C(=O)CN/C(=N\\[C@H](C(=O)N[C@H](C(=O)N[C@H](CC(=O)O)c2nccs2)[C@@H](C)c2ccccc2)C(C)(C)C)[C@H](C(C)(C)C)NC1=O"
                    ],
                    "isIntermediate": true,
                    "description": "P450-catalyzed oxidative decarboxylation of terminal thiazoline of bottromycin precursor."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1021/jacs.0c10361"
                ]
            }
        }
    ]
}