{
    "accession": "MITE0000032",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-3002-6458"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 fixed numbering of reaction SMARTS, removed explicit charges, and reworked substrate/product."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions. Removed non-covalently bond cofactor from reaction depiction to implement editorial guidelines."
        }
    ],
    "enzyme": {
        "name": "SfmM2",
        "description": "C-methyltransferase",
        "references": [
            "doi:10.1128/JB.00826-07"
        ],
        "databaseIds": {
            "uniprot": "B0CN31",
            "genpept": "ABI22137.1",
            "mibig": "BGC0000422"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ],
            "inorganic": [
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Mediates the methylation of tyrosine into 3-methyl-L-tyrosine (3-Me-Tyr) in biosynthesis of saframycin A",
            "reactionSMARTS": "[NH2:1][CH:2]([C:11]([OH:13])=[O:12])[CH2:3][c:4]1[cH:10][cH:9][c:7]([OH:8])[cH:6][cH:5]1>>[NH2:1][CH:2]([C:11]([OH:13])=[O:12])[CH2:3][c:4]1[cH:10][c:9](C)[c:7]([OH:8])[cH:6][cH:5]1",
            "reactions": [
                {
                    "substrate": "NC(Cc1ccc(O)cc1)C(=O)O",
                    "products": [
                        "Cc1cc(CC(N)C(=O)O)ccc1O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1111/j.1365-2958.2004.04433.x"
                ]
            },
            "databaseIds": {
                "rhea": "41428",
                "ec": "2.1.1.304"
            }
        }
    ]
}