{
    "accession": "MITE0000025",
    "status": "retired",
    "retirementReasons": [
        "Entry addresses same enzyme as MITE0000041 (OxyB), but references the wrong MIBiG entry. All information has been transferred to MITE0000041."
    ],
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0009-0008-7609-4484"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 fixed reaction SMARTS formatting. Reviewer 1 retired entry in favor of MITE0000041"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2025-08-26",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Homogenized enzyme descriptions"
        },
        {
            "version": "3",
            "date": "2026-07-02",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Fixed retired UniProtKB accession with UniParc accession."
        }
    ],
    "enzyme": {
        "name": "OxyB",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1016/j.jbiotec.2006.04.009",
            "doi:10.1128/AAC.43.7.1565",
            "doi:10.1002/1521-3773(20011217)40:24%3C4688::aid-anie4688%3E3.0.co;2-m"
        ],
        "databaseIds": {
            "uniprot": "UPI00021436EF",
            "genpept": "AEI58869.1",
            "mibig": "BGC0000311"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ],
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization"
            ],
            "description": "Introduces the first crosslink (biaryl ether) between hpg4 and bht6 of the balhimycin precursor.",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:67])-[#6:3]-[#6:4](-[#7:66])-[#6:5](=[#8:65])-[#7:6]-[#6:7](-[#6:55](-[#8:64])-[#6:56]1:[#6:63]:[#6:62]:[#6:60](-[#8:61]):[#6:58](-[Cl:59]):[#6:57]:1)-[#6:8](=[#8:54])-[#7:9]-[#6:10](-[#6:50]-[#6:51](-[#7:53])=[#8:52])-[#6:11](=[#8:49])-[#7:12]-[#6:13](-[#6:21](=[#8:48])-[#7:22]-[#6:23](-[#6:31](=[#8:47])-[#7:32]-[#6:33](-[#6:37](-[#8:46])-[#6:38]1:[#6:45]:[#6:44]:[#6:42](-[#8:43]):[#6:40](-[Cl:41]):[#6:39]:1)-[#6:34](-[#8:36])=[#8:35])-[#6:24]1:[#6:30]:[#6:29]:[#6:27](-[#8:28]):[#6:26]:[#6:25]:1)-[#6:14]1:[#6:20]:[#6:19]:[#6:17](-[#8:18]):[#6:16]:[#6:15]:1>>[#6:1]-[#6:2](-[#6:67])-[#6:3]-[#6:4](-[#7:66])-[#6:5](=[#8:65])-[#7:6]-[#6:7](-[#6:55](-[#8:64])-[#6:56]1:[#6:63]:[#6:62]:[#6:60](-[#8:61]):[#6:58](-[Cl:59]):[#6:57]:1)-[#6:8](=[#8:54])-[#7:9]-[#6:10](-[#6:50]-[#6:51](-[#7:53])=[#8:52])-[#6:11](=[#8:49])-[#7:12]-[#6:13]1-[#6:21](=[#8:48])-[#7:22]-[#6:23](-[#6:24]2:[#6:25]:[#6:26]:[#6:27](-[#8:28]):[#6:29]:[#6:30]:2)-[#6:31](=[#8:47])-[#7:32]-[#6:33](-[#6:34](-[#8:36])=[#8:35])-[#6:37](-[#8:46])-[#6:38]2:[#6:45]:[#6:44]:[#6:42](:[#6:40](-[Cl:41]):[#6:39]:2)-[#8:43]-[#6:16]2:[#6:17](:[#6:19]:[#6:20]:[#6:14]-1:[#6:15]:2)-[#8:18]",
            "reactions": [
                {
                    "substrate": "CC(C)CC(N)C(=O)NC(C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)O)C(O)c1ccc(O)c(Cl)c1)c1ccc(O)cc1)c1ccc(O)cc1)C(O)c1ccc(O)c(Cl)c1",
                    "products": [
                        "CC(C)CC(N)C(=O)NC(C(=O)NC(CC(N)=O)C(=O)NC1C(=O)NC(c2ccc(O)cc2)C(=O)NC(C(=O)O)C(O)c2ccc(c(Cl)c2)Oc2cc1ccc2O)C(O)c1ccc(O)c(Cl)c1"
                    ],
                    "isIntermediate": true,
                    "description": "The reaction might be less specific than depicted in the reaction SMARTS. There is no detailed study on the specificity so far. Furthermore, the substrate probably needs to be bound to a PCP domain in order to be recognized."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/1521-3773(20011217)40:24%3C4688::aid-anie4688%3E3.0.co;2-m",
                    "doi:10.1016/j.jbiotec.2006.04.009"
                ]
            }
        }
    ]
}