{
    "accession": "MITE0000023",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-3002-6458",
                "0000-0002-9747-3423"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 1 reworked reaction SMARTS by removing partial stereochemistry - reaction passes."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2026-01-02",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added reaction description, cofactors."
        }
    ],
    "enzyme": {
        "name": "SsfM1",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1002/anie.201205426"
        ],
        "databaseIds": {
            "uniprot": "D6MSX0",
            "genpept": "ADE34508.1",
            "mibig": "BGC0000269"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ],
            "inorganic": [
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "O-methylation of tetrycycline intermediate.",
            "reactionSMARTS": "[#6:1]-[#6:2]1-[#6:38](-[#8:39])-[#6:36](-[#8:37])-[#6:35]-[#6:4](-[#6:5]2:[#6:10](-[#8:11]):[#6:9]3-[#6:12](-[#6:14]4-[#6:31](-[#6:32](-[#8:34])(-[#6:33])-[#6:8]:3:[#6:7]:[#6:6]:2)-[#6:30]-[#6:29]2-[#6:17](-[#6:19](-[#6:21](=[#6:25](-[#6:27]-2-[#8:28])-[#8:26])-[#6:22](=[#8:24])-[#7:23])=[#8:20])(-[#8:18])-[#6:15]=4-[#8:16])=[#8:13])-[#8:3]-1>>[#6]-[#8:18]-[#6:17]12-[#6:19](=[#8:20])-[#6:21](-[#6:22](-[#7:23])=[#8:24])=[#6:25](-[#8:26])-[#6:27](-[#8:28])-[#6:29]-1-[#6:30]-[#6:31]1-[#6:14](-[#6:12](=[#8:13])-[#6:9]3:[#6:8](-[#6:32]-1(-[#6:33])-[#8:34]):[#6:7]:[#6:6]:[#6:5](-[#6:4]1-[#6:35]-[#6:36](-[#8:37])-[#6:38](-[#8:39])-[#6:2](-[#6:1])-[#8:3]-1):[#6:10]:3-[#8:11])=[#6:15]-2-[#8:16]",
            "reactions": [
                {
                    "substrate": "CC1OC(c2ccc3c(c2O)C(=O)C2=C(O)C4(O)C(=O)C(C(N)=O)=C(O)C(O)C4CC2C3(C)O)CC(O)C1O",
                    "products": [
                        "COC12C(=O)C(C(N)=O)=C(O)C(O)C1CC1C(=C2O)C(=O)c2c(ccc(C3CC(O)C(O)C(C)O3)c2O)C1(C)O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.201205426"
                ]
            }
        }
    ]
}