{
    "accession": "MITE0000022",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-3002-6458",
                "0000-0002-9747-3423"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer 1 added reference and added reaction description"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "SsfM4",
        "description": "C-methyltransferase",
        "references": [
            "doi:10.1002/anie.201205426",
            "doi:10.1021/ja907852c "
        ],
        "databaseIds": {
            "uniprot": "D6MSU6",
            "genpept": "ADE34484.1",
            "mibig": "BGC0000269"
        },
        "cofactors": {
            "inorganic": [
                "FeS"
            ],
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "SsfM4 catalyzes C6-methylation",
            "reactionSMARTS": "[#7:26]-[#6:24](=[O:25])-[c:16]1[c:17](-[#8:23])[c:18][c:11]2[c:12][c:9]3[c:10][c:1]4[c:2][c:3][c:4][c:5](-[#8:19])[c:6]4[c:7](-[#8:20])[c:8]3[c:13](-[#8:21])[c:14]2[c:15]1-[#8:22]>>[#6]-[c:10]1[c:1]2[c:2][c:3][c:4][c:5](-[#8:19])[c:6]2[c:7](-[#8:20])[c:8]2[c:13](-[#8:21])[c:14]3[c:15](-[#8:22])[c:16](-[#6:24](-[#7:26])=[O:25])[c:17](-[#8:23])[c:18][c:11]3[c:12][c:9]12",
            "reactions": [
                {
                    "substrate": "NC(=O)c1c(O)cc2cc3cc4cccc(O)c4c(O)c3c(O)c2c1O",
                    "products": [
                        "Cc1c2cccc(O)c2c(O)c2c(O)c3c(O)c(C(N)=O)c(O)cc3cc12"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.201205426",
                    "doi:10.1021/ja907852c "
                ]
            }
        }
    ]
}