{
    "accession": "MITE0000015",
    "status": "active",
    "comment": "C-methyltransferase involved in the mithramycin biosynthesis pathway from Streptomyces argillaceus",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-3002-6458",
                "0000-0002-9747-3423"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "Initial entry. Reviewer 2 replaced CXSMARTS with normal SMARTS"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Added cofactor information"
        },
        {
            "version": "3",
            "date": "2026-03-07",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Added minor description details"
        }
    ],
    "enzyme": {
        "name": "MtmMII",
        "description": "C-methyltransferase",
        "references": [
            "doi:10.1074/jbc.275.5.3065"
        ],
        "databaseIds": {
            "uniprot": "Q194P8",
            "genpept": "CAK50790.1",
            "mibig": "BGC0000247"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ],
            "inorganic": [
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "C-methylation in the mithramycin biosynthetic pathway.",
            "reactionSMARTS": "[#6:1]-[#8:2]-[#6:3]1-[#6:58]2-[#6:12](-[#6:42](-[#6:44]3:[#6:55](-[#8:56]):[#6:54]4:[#6:47](:[#6:48]:[#6:49](:[#6:51]:[#6:52]:4-[#8:53])-[#8:50]):[#6:46]:[#6:45]:3-[#6:57]-2)=[#8:43])(-[#8:13]-[#6:14]2-[#8:41]-[#6:39](-[#6:40])-[#6:37](-[#8:38])-[#6:16](-[#8:17]-[#6:18]3-[#8:36]-[#6:34](-[#6:35])-[#6:32](-[#8:33])-[#6:20](-[#8:21]-[#6:22]4-[#8:31]-[#6:29](-[#6:30])-[#6:27](-[#8:28])-[#6:24](-[#8:26])(-[#6:25])-[#6:23]-4)-[#6:19]-3)-[#6:15]-2)-[#6:10](=[#8:11])-[#6:6](-[#6:7](=[#8:9])-[#6:8])=[#6:4]-1-[#8:5]>>[#6:1]-[#8:2]-[#6:3]1-[#6:58]2-[#6:12](-[#6:42](-[#6:44]3:[#6:55](-[#8:56]):[#6:54]4:[#6:47](:[#6:48]:[#6:49](:[#6:51](:[#6:52]:4-[#8:53])-[#6])-[#8:50]):[#6:46]:[#6:45]:3-[#6:57]-2)=[#8:43])(-[#8:13]-[#6:14]2-[#8:41]-[#6:39](-[#6:40])-[#6:37](-[#8:38])-[#6:16](-[#8:17]-[#6:18]3-[#8:36]-[#6:34](-[#6:35])-[#6:32](-[#8:33])-[#6:20](-[#8:21]-[#6:22]4-[#8:31]-[#6:29](-[#6:30])-[#6:27](-[#8:28])-[#6:24](-[#8:26])(-[#6:25])-[#6:23]-4)-[#6:19]-3)-[#6:15]-2)-[#6:10](=[#8:11])-[#6:6](-[#6:7](=[#8:9])-[#6:8])=[#6:4]-1-[#8:5]",
            "reactions": [
                {
                    "substrate": "COC1C(O)=C(C(C)=O)C(=O)C2(OC3CC(OC4CC(OC5CC(C)(O)C(O)C(C)O5)C(O)C(C)O4)C(O)C(C)O3)C(=O)c3c(cc4cc(O)cc(O)c4c3O)CC12",
                    "products": [
                        "COC1C(O)=C(C(C)=O)C(=O)C2(OC3CC(OC4CC(OC5CC(C)(O)C(O)C(C)O5)C(O)C(C)O4)C(O)C(C)O3)C(=O)c3c(cc4cc(O)c(C)c(O)c4c3O)CC12"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1074/jbc.275.5.3065"
                ]
            }
        }
    ]
}