{
    "accession": "MITE0000014",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2024-07-30",
            "contributors": [
                "0000-0003-3002-6458",
                "0000-0002-9747-3423"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "Initial entry. Reviewer 1 added references, checked for factual correctness, added reaction description."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "MtmMI",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1074/jbc.275.5.3065",
            "doi:10.1186/s12934-020-01368-3"
        ],
        "databaseIds": {
            "uniprot": "Q194P7",
            "genpept": "CAK50791.1",
            "mibig": "BGC0000247"
        },
        "cofactors": {
            "inorganic": [
                "FeS"
            ],
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "4-DPMC is the first stable intermediate of the mithramycin biosynthesis. This is converted into PMC by methylation at C-4-O through the MtmMI methyltransferase in Streptomyces argillaceus",
            "reactionSMARTS": "[#6:27]-[#6:26](=[O:28])-[#6:16]-1=[#6:17](-[#8:20])-[#6@@H:18](-[#8:19])-[#6:11]2-[#6:12]-[c:9]3[c:10][c:1]4[c:2][c:3](-[#8:25])[c:4][c:5](-[#8:24])[c:6]4[c:7](-[#8:23])[c:8]3-[#6:13](=[O:22])[C:14]2([#8:29])[#6:15]-1=[O:21]>>[#6]-[#8:19]-[#6@H:18]-1-[#6:11]2-[#6:12]-[c:9]3[c:10][c:1]4[c:2][c:3](-[#8:25])[c:4][c:5](-[#8:24])[c:6]4[c:7](-[#8:23])[c:8]3-[#6:13](=[O:22])[C:14]2([#8:29])[#6:15](=[O:21])-[#6:16](-[#6:26](-[#6:27])=[O:28])=[#6:17]-1-[#8:20]",
            "reactions": [
                {
                    "substrate": "CC(=O)C1=C(O)[C@@H](O)C2Cc3cc4cc(O)cc(O)c4c(O)c3C(=O)C2(O)C1=O",
                    "products": [
                        "CO[C@@H]1C(O)=C(C(C)=O)C(=O)C2(O)C(=O)c3c(cc4cc(O)cc(O)c4c3O)CC12"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1074/jbc.275.5.3065"
                ]
            }
        }
    ]
}